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KMID : 0359319680080022019
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Korean Journal of Veterinary Research
1968 Volume.8 No. 2 p.2019 ~ p.2025
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Studies on the Effects of Some Chemcals on the Germinative Stimulatilon of Clostridiu chauvoei Spore
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Abstract
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The effects of some chemicals for the germinative stimulation of Clostridium chauvoei spore studied. The results obtained were as follows:
1. Cooked meat medium (CMM) was superior to Liver-liver bouillon (LLB) for cultivation of the organisms.
2. Heating the organisms at 70℃ for 30 minutes prior to cultivation in CMM stimulated the germination rate.
3. The addition of 0.5mM L-alanine to CMM was found to be most elective for the rapid germination of the spores.
4. The addition of 0.2 mM D-alanine to CMM inhibited the germination of the spores even if the spores were heat treated.
5. The addition of 0.1mM manganese retarded the germination time, but the final germination rate was greater with 0.1mM manganese than with 0.5mM L-alanine.
6. The addition of both 0.5mM L-alanine and 0.1mM manganese stimulated the germination rate to 100 per cent.
7. The germination rate was greater with the addition of germination stimulants than without any stimulants. The germination rate was approximately 6 per cent greater with prior heat treatment than without heat treatment.
8. The optimum time for the harvest of vegetative forms of the organisms was 15 hours after cultivation in the media which contain suitable germination stimulants.
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